QTL Analysis of Ascochyta Blight Resistance in Chickpea

Ascochyta blight, caused by Ascochyta rabiei(Pass.) Lab. is a devastating disease of chickpea (Cicer arietinum L.) worldwide. Resistant germplasm has been identified and the genetics of resistance has been the subject of numerous studies. Besides,
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  T.-h. Kim et al. (Eds.): FGCN 2008, CCIS 27, pp. 25–40, 2009. © Springer-Verlag Berlin Heidelberg 2009 QTL Analysis of Ascochyta Blight Resistance in Chickpea A. Taleei 1,* , H. Kanouni 2 , and M. Baum 3   1  Professor in the Department of Agronomy & Plant breeding, Faculty of Crop & Animal Sciences, College of Agriculture and Natural Resources, University of Tehran, Karaj, P.O. Box 31587-11167, Iran  2  Seed and Plant Improvement Institute (SPII), 4119-31585, Karaj, Iran 3 International Centre for Agricultural Research in the Dry Areas (ICARDA), Aleppo, Syria Abstract.   Ascochyta blight, caused by  Ascochyta rabiei (Pass.) Lab. is a dev-astating disease of chickpea ( Cicer arietinum L.) worldwide. Resistant germ-plasm has been identified and the genetics of resistance has been the subject of numerous studies. Besides, microsatellites have become markers of choice for molecular mapping and marker assisted selection of key traits such as disease resistance in many crop species. The aim of this study was to construct a ge-netic linkage map and analysis of quantitative trait loci (QTLs) for Ascochyta blight resistance in chickpea ( Cicer arietinum L.), as well as quantify the con-tribution of each QTL to observed phenotypic variation. Fifty-eight SSR markers and one morphological marker (flower color) were mapped on F 2  in-dividuals and F 2:3  families derived from the cross ICC 12004 (resistant) × Bivanij (susceptible local variety) at the International Center for Agricultural Research at Dry Area (ICARDA). The linkage map comprised eight linkage groups, excluding flower color which didn't assign to any linkage group. Area under disease progress curve (AUDPC) was used to evaluate the F 2  population and F 3  families. Using composite interval mapping, three genomic regions were detected, which were in association with reaction to ascochyta blight. These QTLs on LG3, LG4 and LG6 accounted for 46.5% of the total estimated phenotypic variation for reaction to ascochyta blight. Fine mapping of the QTLs identified in this study would lead to the identification of markers that could be used for marker-assisted selection of chickpea genotypes with resis-tance to Ascochyta blight. These findings are particular pertinent considering that we used  Ascochyta rabiei  pathotype III and ICC 12004 (resistant to pathotype III) for the first time. Keywords:  Chickpea (Cicer arietinum L.),  Ascochyta rabiei , Disease resistance, SSR, Linkage map, QTL. *  Corresponding author.
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